Infection of a tomato cell culture by Phytophthora infestans; a versatile tool to study Phytophthora-host interactions.
Identifieur interne : 000978 ( Main/Exploration ); précédent : 000977; suivant : 000979Infection of a tomato cell culture by Phytophthora infestans; a versatile tool to study Phytophthora-host interactions.
Auteurs : Charikleia Schoina [Pays-Bas] ; Klaas Bouwmeester [Pays-Bas] ; Francine Govers [Pays-Bas]Source :
- Plant methods [ 1746-4811 ] ; 2017.
Abstract
BACKGROUND
The oomycete
RESULTS
MsK8 cells can host several
CONCLUSIONS
Our results show that
DOI: 10.1186/s13007-017-0240-0
PubMed: 29090012
PubMed Central: PMC5657071
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Infection of a tomato cell culture by <i>Phytophthora infestans</i>; a versatile tool to study <i>Phytophthora</i>-host interactions.</title><author><name sortKey="Schoina, Charikleia" sort="Schoina, Charikleia" uniqKey="Schoina C" first="Charikleia" last="Schoina">Charikleia Schoina</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Bouwmeester, Klaas" sort="Bouwmeester, Klaas" uniqKey="Bouwmeester K" first="Klaas" last="Bouwmeester">Klaas Bouwmeester</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:29090012</idno><idno type="pmid">29090012</idno><idno type="doi">10.1186/s13007-017-0240-0</idno><idno type="pmc">PMC5657071</idno><idno type="wicri:Area/Main/Corpus">000893</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000893</idno><idno type="wicri:Area/Main/Curation">000893</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000893</idno><idno type="wicri:Area/Main/Exploration">000893</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Infection of a tomato cell culture by <i>Phytophthora infestans</i>; a versatile tool to study <i>Phytophthora</i>-host interactions.</title><author><name sortKey="Schoina, Charikleia" sort="Schoina, Charikleia" uniqKey="Schoina C" first="Charikleia" last="Schoina">Charikleia Schoina</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Bouwmeester, Klaas" sort="Bouwmeester, Klaas" uniqKey="Bouwmeester K" first="Klaas" last="Bouwmeester">Klaas Bouwmeester</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University and Research, Wageningen</wicri:regionArea><wicri:noRegion>Wageningen</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant methods</title><idno type="ISSN">1746-4811</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>The oomycete </p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>MsK8 cells can host several </p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Our results show that </p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29090012</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1746-4811</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Plant methods</Title><ISOAbbreviation>Plant Methods</ISOAbbreviation></Journal><ArticleTitle>Infection of a tomato cell culture by <i>Phytophthora infestans</i>; a versatile tool to study <i>Phytophthora</i>-host interactions.</ArticleTitle><Pagination><MedlinePgn>88</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13007-017-0240-0</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The oomycete <i>Phytophthora infestans</i> causes late blight on potato and tomato. Despite extensive research, the <i>P.</i> <i>infestans</i>-host interaction is still poorly understood. To find new ways to further unravel this interaction we established a new infection system using MsK8 tomato cells. These cells grow in suspension and can be maintained as a stable cell line that is representative for tomato.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">MsK8 cells can host several <i>Phytophthora</i> species pathogenic on tomato. Species not pathogenic on tomato could not infect. Microscopy revealed that 16 h after inoculation up to 36% of the cells were infected. The majority were penetrated by a germ tube emerging from a cyst (i.e. primary infection) while other cells were already showing secondary infections including haustoria. In incompatible interactions, MsK8 cells showed defense responses, namely reactive oxygen species production and cell death leading to a halt in pathogen spread at the single cell level. In compatible interactions, several <i>P.</i> <i>infestans</i> genes, including RXLR effector genes, were expressed and in both, compatible and incompatible interactions tomato genes involved in defense were differentially expressed.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Our results show that <i>P.</i> <i>infestans</i> can prosper as a pathogen in MsK8 cells; it not only infects, but also makes haustoria and sporulates, and it receives signals that activate gene expression. Moreover, MsK8 cells have the ability to support pathogen growth but also to defend themselves against infection in a similar way as whole plants. An advantage of MsK8 cells compared to leaves is the more synchronized infection, as all cells have an equal chance of being infected. Moreover, analyses and sampling of infected tissue can be performed in a non-destructive manner from early time points of infection onwards and as such the MsK8 infection system offers a potential platform for large-scale omics studies and activity screenings of inhibitory compounds.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y" EqualContrib="Y"><LastName>Schoina</LastName><ForeName>Charikleia</ForeName><Initials>C</Initials><Identifier Source="ORCID">0000-0001-5840-9683</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</Affiliation><Identifier Source="ISNI">0000 0001 0791 5666</Identifier><Identifier Source="GRID">grid.4818.5</Identifier></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Bouwmeester</LastName><ForeName>Klaas</ForeName><Initials>K</Initials><Identifier Source="ORCID">0000-0002-8141-3880</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</Affiliation><Identifier Source="ISNI">0000 0001 0791 5666</Identifier><Identifier Source="GRID">grid.4818.5</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Govers</LastName><ForeName>Francine</ForeName><Initials>F</Initials><Identifier Source="ORCID">0000-0001-5311-929X</Identifier><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands.</Affiliation><Identifier Source="ISNI">0000 0001 0791 5666</Identifier><Identifier Source="GRID">grid.4818.5</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>10</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Methods</MedlineTA><NlmUniqueID>101245798</NlmUniqueID><ISSNLinking>1746-4811</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cell death</Keyword><Keyword MajorTopicYN="N">Defense responses</Keyword><Keyword MajorTopicYN="N">Disease</Keyword><Keyword MajorTopicYN="N">Gene expression</Keyword><Keyword MajorTopicYN="N">Infection</Keyword><Keyword MajorTopicYN="N">Microscopy</Keyword><Keyword MajorTopicYN="N">MsK8</Keyword><Keyword MajorTopicYN="N">Reactive oxygen species (ROS)</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>09</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>10</Month><Day>17</Day></PubMedPubDate><PubMedPubDate 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name="Pays-Bas"><noRegion><name sortKey="Schoina, Charikleia" sort="Schoina, Charikleia" uniqKey="Schoina C" first="Charikleia" last="Schoina">Charikleia Schoina</name></noRegion><name sortKey="Bouwmeester, Klaas" sort="Bouwmeester, Klaas" uniqKey="Bouwmeester K" first="Klaas" last="Bouwmeester">Klaas Bouwmeester</name><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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